Degree in Medical
Biotechnology - University of Bologna
ADVANCED
HUMAN GENETICS (I.C.)
Module
of CLASSICAL AND NEXT-GENERATION GENOMICS
- A.Y. 2023/2024
Teacher: Pierluigi
Strippoli
Teaching Material 2023/2024 in Virtuale
Paper vs. Pixels
1.
Human
Genomics
[Source for the exam: Chapters #1, #2 and #3 only from
Information - Gene -
Genome Lecture
Notes in Virtuale]
2. Bibliographic
Databases
3. Nucleic
Acid Extraction
[Source
for the exam:
Nucleic
Acids Extraction principles]
4. Nucleic
Acid
Hybridization -
Concept of Probe
Specificity - Southern
Blot - Northern
Blot - Microarray
[Source
for the exam:
Nucleic
Acid
Hybridization principles;
and the
section "Principle
of method" in this
review: Expression
Profiling]
5. Polymerase
Chain Reaction
(PCR)
- Primer design [Example
Article] -
Reaction
Kinetics
[Source
for the exam: PCR
principles]
6. Genetic
Libraries - RNA
Vaccine
[Source for the exam: EST
libraries principles;
and sections
nn. 1
and 2
in this
review: The 5´ end mRNA artifact in cDNA cloning]
7. Nucleic
Acid Sequencing
and Sequence
Analysis
[Source
for the exam: Classic
DNA Sequencing notes]
[Source
for the exam: Bioinformatics
Analysis in
Genomics principles;
and Sequence
Alignment and Sequence
Search
articles in
Virtuale]
8. System
Biology -
The case of trisomy
21: a model for
integrated research
[Source for the exam: sections
nn. 1, 2, 3,
7, 10, 11, 12 in
this review: Genetics
and Genomics
of Down
Syndrome in
Virtuale]
FILMOGRAPHY
Ron Howard
(Director). Apollo
13.
With Tom Hanks,
Ed Harris. USA,
1995.
["Houston, we
have a problem".
A true story.
The positive
hypothesis when
dealing with a
problem].
George
Miller
(Director). Lorenzo's
Oil.
With Nick Nolte,
Susan Sarandon.
USA, 1992.
[The true story
of two parents
of a child with
adrenoleukodystrophy -
X-linked disease
- who discover a
diet therapy].
Tom Vaughan
(Director). Extraordinary
Measures.
With Harrison
Ford, Brendan
Fraser. USA,
2010.
[The story,
inspired by
reality, of the
discovery of a
cure for Pompe disease (Glycogenosis
Type II)].
Andrew Niccol
(Director). Gattaca.
With Ethan
Hawke, Uma
Thurman. USA,
1997.
["There is no
gene for the
human spirit."].
***
Laboratory
Lab 1 - Data
Banks
Lab 2 - Gene Structure - Lab
Exercise
Lab 3 - PCR
Primer - Example Article (MTHFR) - Example
Article [COVID-19] - In-Silico PCR
Lab 4 - Data
Analysis
Lab 5 - Documentary
DNA