DNA > Biotech

Genetic Libraries

0.	Cloning: concept
 
1.	Vectors
	- Replication origin	(Copy number)
	- Cloning site (polylinker)	 (ENDS)
	- Selection marker - Blue/White
	- Example

2.	Genetic Libraries - Expressed Sequence Tags - EST - dbEST - EST BE969574 
	UniGene - Gene

3.	The 5´ Problem
	Rapid Amplification of cDNA Ends - RACE)
	(T4RL; TAP)

	Human 5´ coding region completeness

DNA