Gene
expression in H. sapiens - An example
http://apollo11.isto.unibo.it/
1. mRNA as a vaccine.
(from "The courage of Edward Jenner, by Mino Milani and Dino
Battaglia,
Corriere dei Ragazzi, n. 27, 1972)
2. Sequence of the SARS-CoV-2 vaccine (BioNTech/Pfizer):
(.docx)
(.pdf)
3. Sequence of the SARS-CoV-2
viral genome (Wuhan-Hu-1),
and of the spike
protein (YP_009724390) (gene:
surface
glycoprotein)
4. Replace Ψ with T.
Discuss the meaning of the various regions of
the mRNA.
Analyze the RNA structure.
BLASTN
2 Seq BLASTN
BLASTP
2 Seq
BLASTP
BLASTX
2 Seq
(deselect "low complexity regions")
BLASTX
ATGTTTGTTTTTCTTGTTTTA Virus
ATGTTCGTGTTCCTGGTGCTG
Vaccine
M . .
Appendix
1. Kozak sequence
GCCRCCAUGG
2. Genetics Code
3. Codon bias - Codon
Usage Frequency Table - Codon Usage Database
Rhinolophus acuminatus (Bat)
Paguma larvata (Himalayan
Civet)
Nyctereutes procyonoides (Raccoon)
Documents
mRNA
Technology Gave Us the First COVID-19 Vaccines (Time,
January 11, 2021)
2023 Nobel
Prize lectures in physiology or medicine | Katalin Karikó and
Drew Weissman (2023, December 7)
[Screenshot]
Il
labirinto del vaccino: le ricerche di Weissman e Karikò e
l’intrigo internazionale sulla cura anti Covid
(Corriere della Sera, 7 Dicembre 2020)
Solutions