Molecular Biology Methods

Sources · Safety · Guidelines · Basic · Microscopy ·Cell culture ·DNA/RNA isolation · Electrophoresis · Purification · Recombinant DNA · PCR · Sequencing
Cytogenetics  · Linkage · Gene Therapy · Functional Genomics · DNA Arrays

General sources about Molecular Biology Methods

Books about Molecular Biology methods:
Molecular Cloning: a Laboratory Manual, by J. Sambrook and D. Russell, CSHL Press, Third Edition, 2000
"Current" Protocols in Molecular Biology, by F.M. Ausubel et al., Wiley, 1988-2000

Online Methods Sources:


BIOSCI Newsgroups
  • BIOSCI METHDS-REAGNTS (a valuable discussion group about Methods and Reagents in Molecular Biology)
  • Safety

    Laboratory practice guidelines

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    Basic


    Statistic Analysis: GraphPad Library   |   How to choose a statistical test

    Microscopy

  • Laboratorio di Istopatologia - Ospedale Sant'Orsola-Malpighi (Ita)
  • Cell biology

    DNA/RNA isolation

    Nucleic Acids Electrophoresis - Blots

    DNA gel purification

    Recombinant DNA Technology

  • CloneIt!- an online program finding sub-cloning strategies
  • Molecular Biology Programs - EnzymeX, a digestion simulator for MacOS X

  • Common vectors:
  • pT7T3D-PAC
  • pCS2 Plasmid - Map (Word file) http://bioinformatics.org/JaMBW/
  • PCR

    DNA sequencing


    Cytogenetics

    Linkage Analysis

    Gene therapy


    Functional Genomics

    Arrays of DNA General - Consumables · Software: · Image,Â¥Data,Â¥Analysis - Ontology

    ARRAYS - CONSUMABLES  General - Software: · Image, Data, Analysis - Ontology
    SOFTWARE - GENERAL  General - Consumables · Software: · Image, Data, Analysis - Ontology
    SOFTWARE - ARRAY IMAGEANALYSIS General - Consumables · Software: · Image, Data, Analysis - Ontology
    SOFTWARE - ARRAY DATA FORMAT, LANGUAGES AND DATABASES
    General - Consumables · Software: · Image, Data, Analysis - Ontology
    SOFTWARE - ARRAY DATA ANALYSIS AND CLUSTERING
    General - Consumables· Software: · Image,Â¥Data, Analysis - Ontology  
    ONTOLOGYÂ¥General - Consumables · Software: · Image, Data, Analysis - Ontology
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    NEURAL NETWORKS AND MICROARRAYS
    NANOTECHNOLOGY

    Methylome

    Next Generation Sequencing



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    Last updated May 28, 2011

    DNA