Human c-kit gene Exon 12 amplification (35 cycles).

Lanes 1-3: DNA   125 ng; primer 0.1, 0.4, and 1.4 microM, respectively.
Lanes 4-6: DNA 1250 ng; primer 0.1, 0.4, and 1.4 microM, respectively.
Lane 7: negative control (water).
Lane M: Marker (Marker V, 5 microL).